A combined approach for the localization and tandem affinity purification of protein complexes from metazoans.

@article{Cheeseman2005ACA,
  title={A combined approach for the localization and tandem affinity purification of protein complexes from metazoans.},
  author={Iain M Cheeseman and Arshad Desai},
  journal={Science's STKE : signal transduction knowledge environment},
  year={2005},
  volume={2005 266},
  pages={pl1}
}
An understanding of a protein's function is greatly aided by the knowledge of its localization in vivo and identification of any interacting partners. Here, we describe a method, based on expression of a genetically encoded fusion protein, that allows for protein localization and affinity purification (LAP) in metazoans. This method makes it possible to rapidly identify transformants and to conduct live imaging of protein localization and dynamics. In addition, the same tag can be used in a… CONTINUE READING

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