A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants.

@article{Sule2009ACO,
  title={A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants.},
  author={Preeti Sule and Tanush Wadhawan and Norman J Carr and Shelley M. Horne and Alan J Wolfe and Birgit M. Pr{\"u}\ss},
  journal={Letters in applied microbiology},
  year={2009},
  volume={49 3},
  pages={299-304}
}
AIMS The aim of this study was to develop an assay system that can quantify the amount of biomass in biofilms formed by different isogenic mutants of an Escherichia coli K-12 strain. METHODS AND RESULTS The reported assay, which is based on the BacTiter-Glo assay from Promega, uses bioluminescence to detect the intracellular concentration of ATP, which correlates with viable bacterial cell numbers. The quantitative data obtained with this ATP assay were compared to those obtained with the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 21 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Use of the BacTiter-Glo microbial cell viability assay to study bacterial attachment in biofilm formation

  • P. Sule, T. Wadhawan, A. J. Wolfe, B. M. Prüß
  • Promega Notes
  • 2008
1 Excerpt

Similar Papers

Loading similar papers…