A colorimetric method for determination of γ-glutamyl-S-ethenyl-cysteine in narbon vetch (Vicia narbonensis L.) seeds.

Abstract

A new colorimetric method based on the bleaching of the iodoplatinate ion has been developed for fast and easy determination of γ-glutamyl-S-ethenyl-cysteine (GEC) in narbon vetch (Vicia narbonensis L.) seeds. The calibration curve showed a good correlation (r(2)=0.9959) between absorbance and GEC amounts from 5.5 to 33 μg (10-59.78 μmol/L). The limits of detection and quantification were 1.16 and 3.55 μmol/L, respectively, and no significant interferences from other sulfur-containing compounds were observed. The method showed excellent repeatability (relative standard deviation [RSD]=0.28%), reproducibility (RSD=4.4%), and accuracy (94%). Determination of GEC in 20 narbon vetch accessions yielded values that were in agreement with those reported previously using capillary electrophoresis and high-performance liquid chromatography methods. The method could be especially valuable for determination of GEC during the process of production of new low-GEC narbon vetch varieties.

DOI: 10.1016/j.ab.2011.07.010

Cite this paper

@article{SnchezVioque2011ACM, title={A colorimetric method for determination of γ-glutamyl-S-ethenyl-cysteine in narbon vetch (Vicia narbonensis L.) seeds.}, author={Ra{\'u}l S{\'a}nchez-Vioque and Mar{\'i}a Fernanda Rodr{\'i}guez-Conde and Javier Vioque and Julio Gir{\'o}n-Calle and Omar Santana-M{\'e}ridas and Marcelino De-Los-Mozos-Pascual and M E Izquierdo-Melero and Manuel Alaiz}, journal={Analytical biochemistry}, year={2011}, volume={418 2}, pages={180-3} }