A colorimetric assay for steady-state analyses of iodo- and bromoperoxidase activities.

@article{Verhaeghe2008ACA,
  title={A colorimetric assay for steady-state analyses of iodo- and bromoperoxidase activities.},
  author={Elodie Françoise Verhaeghe and David H. Buisson and Elisabeth Z{\'e}kri and Catherine Leblanc and Philippe Potin and Yves Ambroise},
  journal={Analytical biochemistry},
  year={2008},
  volume={379 1},
  pages={60-5}
}
The standard assay for iodoperoxidase activity is based on the spectrophotometric detection of triiodide formed during the enzymatic reaction. However, chemical instability of I3- has limited the method to high iodide concentrations and acidic conditions. Here we describe a simple spectrophotometric assay for the determination of iodoperoxidase activities of vanadium haloperoxidases based on the halogenation of thymol blue. The relation between color and chemical entities produced by the vHPO/H… CONTINUE READING
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