A color-based stable multi-copy integrant selection system for Pichia pastoris using the attenuated ADE1 and ADE2 genes as auxotrophic markers.

@article{Du2012ACS,
  title={A color-based stable multi-copy integrant selection system for Pichia pastoris using the attenuated ADE1 and ADE2 genes as auxotrophic markers.},
  author={Min Du and Michael Benjamin Battles and Juergen Hermann Nett},
  journal={Bioengineered bugs},
  year={2012},
  volume={3 1},
  pages={
          32-7
        }
}
  • Min Du, Michael Benjamin Battles, Juergen Hermann Nett
  • Published in Bioengineered bugs 2012
  • Biology, Medicine
  • The methylotropic yeast Pichia pastoris has been used for more than two decades to successfully produce a large number of recombinant proteins. Currently, a wide variety of auxotrophic and drug based selection markers are employed to screen for clones expressing the protein of interest. For most proteins an increased copy number of the integrated plasmid results in higher levels of expression, but these multi-copy integrants can be unstable due to the propensity of P. pastoris for homologous… CONTINUE READING

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