A colony hybridization study of mRNA in maturating soybean seeds


Polysomal poly(A)RNA was transcribed by avian myeloblastosis virus (AMV) reverse transcriptase and double-stranded complementary DNA (cDNA) was then synthesized and cloned in Escherichia coli C600 by (dG)-(dC) tailing in the PstI site of pBR322. A bank of mRNA sequences from maturating soybean seeds (average weight 200 mg) was obtained. 32P-cDNA based on… (More)
DOI: 10.1007/BF00272636


1 Figure or Table