A colony hybridization study of mRNA in maturating soybean seeds

Abstract

Polysomal poly(A)RNA was transcribed by avian myeloblastosis virus (AMV) reverse transcriptase and double-stranded complementary DNA (cDNA) was then synthesized and cloned in Escherichia coli C600 by (dG)-(dC) tailing in the PstI site of pBR322. A bank of mRNA sequences from maturating soybean seeds (average weight 200 mg) was obtained. 32P-cDNA based on… (More)
DOI: 10.1007/BF00272636

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