A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome

@article{Clarke1976ACB,
  title={A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome},
  author={Louise A Clarke and John R Carbon},
  journal={Cell},
  year={1976},
  volume={9},
  pages={91-99}
}
Using the poly(dA-dT) "connector" method (Lobbanand Kaiser, 1973), a population of annealed hybrid circular DNAs was constructed in vitro; each hybrid DNA circle contained one molecule of poly(dT)-tailed Col El-DNA (LRI) annealed to any one of a collection of poly(dA)-tailed linear DNA fragments, produced originally by shearing total E. coli DNA to an average size of 8.5 x 10(6) daltons. This annealed DNA preparation (12 mug) was used to transform an F+ recA E. coli strain (JA200), selecting… CONTINUE READING
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