A colonisation-inhibition culture consisting of Salmonella Enteritidis and Typhimurium ΔhilAssrAfliG strains protects against infection by strains of both serotypes in broilers.

  title={A colonisation-inhibition culture consisting of Salmonella Enteritidis and Typhimurium $\Delta$hilAssrAfliG strains protects against infection by strains of both serotypes in broilers.},
  author={W De Cort and Dorien Mot and Freddy Haesebrouck and Richard Ducatelle and Filip Van Immerseel},
  volume={32 36},
Consumption of contaminated poultry meat is still an important cause of Salmonella infections in humans and there is a need for control methods that protect broilers from day-of-hatch until slaughter age against infection with Salmonella. Colonisation-inhibition, a concept in which a live Salmonella strain is orally administered to day-old chickens and protects against subsequent challenge, can potentially be used as control method. In this study, the efficacy of a Salmonella Typhimurium… Expand
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Prevention of egg contamination by Salmonella Enteritidis after oral vaccination of laying hens with Salmonella Enteritidis ΔtolC and ΔacrABacrEFmdtABC mutants
The efficacy of Salmonella Enteritidis ΔtolC and ΔacrABacrEFmdtABC strains in laying hens as live vaccines was evaluated, and they significantly protected against gut and internal organ colonization, and completely prevented egg contamination by Salmonellosis under the conditions of this study. Expand
rOmpF and OMVs as efficient subunit vaccines against Salmonella enterica serovar Enteritidis infections in poultry farms.
Both the expressed recombinant outer membrane protein F (rOmpF) and extracted outer membrane vesicles (OMVs) were developed as subunit vaccines against S. Enteritidis challenge in chickens and revealed the potential application of r ompF and OMVs as sub unit vaccines in the poultry industry. Expand
Current and Future Perspectives on Development of Salmonella Vaccine Technologies
This chapter provides an overview of the use of Salmonella vaccines for poultry to prevent food-borne zoonosis of concern for public health and of the recent advances in a new generation ofSalmonella -vectored multivalent vaccines used for delivery of protective antigens for immunization against pathogens to benefit poultry health. Expand
Flagellin and multidrug resistance mutants as future Salmonella vaccines for laying hens
In deze thesis werd nagegaan of de Salmonella Typhimurium stam Nal2/Rif9/Rtt, die aanwezig is in de commercieel beschikbare levende vaccins AviPro® Salmonell Duo en Avi pro®, Salmoneella VacT ook bescherming biedt tegen deze monofasische variant. Expand


A Salmonella Enteritidis hilAssrAfliG deletion mutant is a safe live vaccine strain that confers protection against colonization by Salmonella Enteritidis in broilers.
This is the first report of an attenuated Salmonella strain for which both the safety and efficacy has been shown in long-term experiments (until slaughter age) in broiler strain can potentially be used as a live colonization-inhibition strain for controllingSalmonella Enteritidis infections in broilers. Expand
Intestinal colonisation-inhibition and virulence of Salmonella phoP, rpoS and ompC deletion mutants in chickens.
Administration of live Salmonella strains to day-old chicks provides profound protection against superinfection with a related strain within a matter of hours by a colonisation-inhibition mechanism,Expand
Long-term colonisation-inhibition studies to protect broilers against colonisation with Salmonella Enteritidis, using Salmonella Pathogenicity Island 1 and 2 mutants.
Protection lasted for the full 6 weeks of study, but due to their persistence, the sipA and ssrA mutants are not useful to induce broiler protection. Expand
Exploitation of Intestinal Colonization‐Inhibition Between Salmonella Organisms for Live Vaccines in Poultry – Potential and Limitations
Treatment of young chicks with mixtures of different Salmonella serovar resulted not only in a very strong growth inhibition of the isogenic strains but also in a substantial inhibition of heterologous serovars, suggesting the potential to exploit this phenomenon in the development of novel live S. Enteritidis vaccines. Expand
Inhibition of colonization of the chicken alimentary tract with Salmonella typhimurium gram-negative facultatively anaerobic bacteria.
Oral administration of strains of food poisoning salmonellas to day-old chickens produced a profound inhibition in the subsequent colonization of the caeca by a strain of Salmonella typhimurium given one day later, and this inhibition was reproduced in vitro by mixed culture experiments. Expand
The effect of oral administration of a homologous hilA mutant strain on the long-term colonization and transmission of Salmonella Enteritidis in broiler chickens.
The observed colonization-inhibition (CI) indicates that administration of live attenuated hilA mutant Salmonella strains to newly hatched chicks might, in combination with other protective control measures, contribute to the control ofSalmonella infections in broilers. Expand
Vaccination and early protection against non-host-specific Salmonella serotypes in poultry: exploitation of innate immunity and microbial activity
It is recommended that all live vaccines considered for oral administration should be tested for their ability to induce the two protective effects described above, including specific colonization-inhibition (competitive exclusion) in which the protective bacteria exert a profound resistance to establishment and colonization by other related bacteria. Expand
Further studies on the inhibition of colonization of the chicken alimentary tract with Salmonella typhimurium by pre-colonization with an avirulent mutant.
Oral administration to newly hatched chickens or to chicks up to 5 days of age with an avirulent, rough, spectinomycin-resistant mutant of Salmonella typhimurium strain F98 inhibited the colonizationExpand
Salmonella Typhimurium induces SPI-1 and SPI-2 regulated and strain dependent downregulation of MHC II expression on porcine alveolar macrophages
It is found that Salmonella Typhimurium strain 112910a specifically downregulated MHC II, but not MHC I, expression on porcine alveolar macrophages in aSalmonella pathogenicity island (SPI)-1 and SPI-2 dependent way. Expand
Experimental oral infection of specific pathogen-free laying hens and cocks with Salmonella enteritidis strains.
It seems that primary vertical transmission of salmonellas occurs, in rare cases, as a result of an infection of the albumen but not of the yolk, in the groups that included salmonella-infected cocks. Expand