A colonic mineralocorticoid receptor cell model expressing epithelial Na+ channels.

@article{Bergann2009ACM,
  title={A colonic mineralocorticoid receptor cell model expressing epithelial Na+ channels.},
  author={Theresa Bergann and Svenja Ploeger and Anja Fromm and Sebastian Zeissig and Steffen A Borden and Michael Fromm and J{\"o}rg-Dieter Schulzke},
  journal={Biochemical and biophysical research communications},
  year={2009},
  volume={382 2},
  pages={280-5}
}
In the distal colon, the epithelial sodium channel (ENaC) is rate limiting for sodium absorption. Progress in the molecular characterization of ENaC expression and trafficking in response to the mineralocorticoid aldosterone has been hampered, since no epithelial colonic cell line existed expressing functional ENaC stimulated by nanomolar aldosterone via mineralocorticoid receptor (MR). Here, we present a human colonic epithelial cell line inducibly expressing the MR (HT-29/B6-Tet-On-MR) which… CONTINUE READING