A colloidal gold labeling technique for the direct determination of the surface area of eukaryotic cells.

@article{Kehle1989ACG,
  title={A colloidal gold labeling technique for the direct determination of the surface area of eukaryotic cells.},
  author={T Kehle and Volker Herzog},
  journal={European journal of cell biology},
  year={1989},
  volume={48 1},
  pages={19-26}
}
We have developed a colloidal gold labeling technique for the direct quantitation of the cell surface area. The method is based on coating the cell surface with [195Au] colloidal gold-protein complexes followed by morphometric determination of the labeling density (gold particles/micron2 cell surface) and radiometric determination of the total number of gold particles bound per cell. The ratio of both values directly gives the cell surface area. The accuracy of the method was shown using… CONTINUE READING