A co-transformation system to produce transgenic grapevines free of marker genes

@inproceedings{Dutt2008ACS,
  title={A co-transformation system to produce transgenic grapevines free of marker genes},
  author={Manjul Dutt and Zhijian T. Li and Sadanand A. Dhekney and Dennis J. Gray},
  year={2008}
}
A co-transformation system was developed to produce grapevines free of selectable marker genes. This was achieved by transforming Vitis vinifera L. ‘Thompson Seedless’ somatic embryos with a mixture of two Agrobacterium strains. The first strain contained a binary plasmid with an egfp gene of interest between the T-DNA borders. The second strain harbored the neomycin phosphotransferase (nptII) gene for positive selection and the cytosine deaminase (codA) gene for negative selection, linked… CONTINUE READING