A cluster of three genes responsible for allantoin degradation in Saccharomyces cerevisiae.

  title={A cluster of three genes responsible for allantoin degradation in Saccharomyces cerevisiae.},
  author={Terrance G. Cooper and M. G{\'o}rski and Vanessa Turoscy},
  volume={92 2},
Mutant strains of Saccharomyces cerevisiae unable to utilize allantoin as sole nitrogen source were isolated and divided into three groups on the basis of their biochemical and genetic characteristics. The three loci associated with these mutant classes were designated dal1 (allantoinase minus), dal2 (allantoicase minus) and dal4 (allantoin transport minus). All three loci are located in a cluster that is proximal to the lys1 locus on the right arm of chromosome IX. The gene order and… 
The allantoinase (DAL1) gene of Saccharomyces cerevisiae
Observations further support the roles of UASNTR and DAL7 UIS in the regulation of allantoin pathway gene expression.
Regulation of allantoate transport in wild-type and mutant strains of Saccharomyces cerevisiae
The pleiotropic behavior of the dal4 and dal5 mutations are interpreted as deriving from a functional interaction between elements of the two transport systems, which appear to be sensitive to nitrogen catabolite repression, feedback inhibition, and trans-inhibition.
Pleiotropic control of five eucaryotic genes by multiple regulatory elements
It is concluded that dal81 mutant strains possess a defect in the induction of enzyme synthesis; enzyme production due to relief of nitrogen catabolite repression, however, appears normal.
Allantoin transport in Saccharomyces cerevisiae is regulated by two induction systems
Induction is probably a transcriptionally regulated process, because addition of hydantoin acetate or oxalurate to the culture medium increased the steady-state levels of mRNA encoded by a gene required for allantoin transport (DAL4).
The allantoin and uracil permease gene sequences of Saccharomyces cerevisiae are nearly identical
The finding of these homologous sequences predicted to exist on the basis of DAL4's expression characteristics, supports and strengthens the suggestion that these elements mediate the functions the authors have previously ascribed to them.
Genetic and biochemical characterization of the galactose gene cluster in Kluyveromyces lactis
The genetic basis of this constitutive gene expression was investigated and found no recombinants between the constitutive and Gal- phenotypes among 76 tetrads, suggesting that either GAL7 or a tightly linked gene codes for a regulatory function.
Clustering of genes for 20 kd zein subunits in the short arm of maize chromosome 7.
Evidence that the structural genes coding for the 20 kd proteins are clustered on the short arm of chromosome 7, a region that also bears loci regulating endosperm zein biosynthesis, suggests that the evolution of at least some of the maize genome has occurred as the result of repeated duplication and divergence of chromosome segments.
Functional Expression and Characterization of the Two Cyclic Amidohydrolase Enzymes, Allantoinase and a Novel Phenylhydantoinase, from Escherichia coli
Despite the unclear physiological function of HyuA, its presence, along with the allantoin-utilizing AllB, strongly suggested that the cyclic ureides might be utilized as nutrient sources in E. coli.


A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae.
More detailed investigations in Neurospora have revealed that the hist-3 region controls three steps in histidine biosynthesis and appears to be partially analogous to the more extensive histidine operon in Salmonella.
Induction of the allantoin degradative enzymes in Saccharomyces cerevisiae by the last intermediate of the pathway.
  • T. Cooper, R. Lawther
  • Biology, Chemistry
    Proceedings of the National Academy of Sciences of the United States of America
  • 1973
It is shown that allophanic acid is required for synthesis of all enzymes participating in allantoin degradation, based upon the observation that wild-type strains produced a large amount ofallantoinase upon addition of allantoine, allantoate, ureidoglycolate, or urea to the medium.
Nitrogen Repression of the Allantoin Degradative Enzymes in Saccharomyces cerevisiae
It is concluded that repression previously attributed to ammonia may result from its metabolism to amino acids and other metabolites.
The frequency in Neurospora tetrads of multiple exchanges within short intervals
Evidence on whether meiotic exchanges that the geneticist has conventionally called single are really simple, or whether they may actually consist of clusters of exchanges within short regions of effective pairing is provided by tetrad data from short areas of the genetic map.
for allantoin degradation in Saccharomyces cereuisiae
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degradative enzymes in Saccharomyces cereuisiae
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Repression and derepression of allantoinase synthesis in protoplasts of Saccharomyces carbergensis. I. Characteristics of the enzyme and introductory experiments on the derpression of its synthesis
  • Prac. K. Ned. Akad. Wet. Ser. B
Genetics Clustering of the genes the determination of glyoxylic acid
  • Bipartite structure of the a&? locus of Saccharomyces cereuisiae
Assay methods for key enzymes of the glyoxylate cycle
  • Biochem. J
  • 1959