A closed tube format for amplification and detection of DNA based on energy transfer.

@article{Nazarenko1997ACT,
  title={A closed tube format for amplification and detection of DNA based on energy transfer.},
  author={Irina Nazarenko and Satish K. Bhatnagar and R J Hohman},
  journal={Nucleic acids research},
  year={1997},
  volume={25 12},
  pages={2516-21}
}
A new method for the direct detection of PCR-amplified DNA in a closed system is described. The method is based on the incorporation of energy transfer-labeled primers into the amplification product. The PCR primers contain hairpin structures on their 5'ends with donor and acceptor moieties located in close proximity on the hairpin stem. The primers are designed in such a way that a fluorescent signal is generated only when the primers are incorporated into an amplification product. A signal to… CONTINUE READING
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References

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Annu. Rev. Biophys. Biomol. Struct

  • G Varani
  • Annu. Rev. Biophys. Biomol. Struct
  • 1995

Methods Enzymol

  • P R Selvin
  • Methods Enzymol
  • 1995

Nature Biotechnol

  • S Tyagi, F R Kramer
  • Nature Biotechnol
  • 1995

Proc. Natl. Acad. Sci. USA

  • J Ju, C Ruan, C W Fuller, A N Glazer, R A Mathies
  • Proc. Natl. Acad. Sci. USA
  • 1995

Nucleic Acids Res

  • G L Lee, C R Connell, W Bloch
  • Nucleic Acids Res
  • 1993

Science

  • V Lyamichev, M A D Brow, J E Dahlberg
  • Science
  • 1993

Trends Genet

  • U Landegren
  • Trends Genet
  • 1993

Gene Nucleic Acids Res

  • M C Longo, M S Berninger, J L Hartley
  • Gene Nucleic Acids Res
  • 1990

PCR Protocols. A Guide to Methods and Applications

  • C Orrego
  • PCR Protocols. A Guide to Methods and…
  • 1990

Tetrahedron Lett

  • G T Wang, E D Matayoshi, H J Huffaker, G A Krafft
  • Tetrahedron Lett
  • 1990

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