A closed-tube detection of loop-mediated isothermal amplification (LAMP) products using a wax-sealed fluorescent intercalator.

Abstract

LAMP is an isothermal amplification method that can achieve ultra-high sensitivity and specificity. However, the conventional detection of LAMP amplicons can lead to cross-contamination due to the need to open the reaction tube which contains a large number of amplicons. To achieve closed-tube LAMP detection, we have developed a method that separates a… (More)

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Cite this paper

@article{Liang2013ACD, title={A closed-tube detection of loop-mediated isothermal amplification (LAMP) products using a wax-sealed fluorescent intercalator.}, author={Chao Liang and Sijia Cheng and Ya’nan Chu and Haiping Wu and Binjie Zou and Huan Huang and Tao Xi and Guohua Zhou}, journal={Journal of nanoscience and nanotechnology}, year={2013}, volume={13 6}, pages={3999-4005} }