A clone screening method using mRNA levels to determine specific productivity and product quality for monoclonal antibodies.

@article{Lee2009ACS,
  title={A clone screening method using mRNA levels to determine specific productivity and product quality for monoclonal antibodies.},
  author={Christina J Lee and Gargi Seth and Joni Tsukuda and Robert B. Hamilton},
  journal={Biotechnology and bioengineering},
  year={2009},
  volume={102 4},
  pages={1107-18}
}
To meet increasing demands for efficient and streamlined production processes of therapeutic antibodies, improved methods of screening clones are required. In this article, we examined the potential of using antibody transcript levels as criteria for clone screening. We evaluated the QuantiGene Plex, a commercially available, high-throughput assay for simultaneously measuring multiple transcripts from cell lysate. Using the development of stable Chinese hamster ovary cell lines as examples, we… CONTINUE READING

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