A cis-antisense RNA acts in trans in Staphylococcus aureus to control translation of a human cytolytic peptide

@article{Sayed2012ACR,
  title={A cis-antisense RNA acts in trans in Staphylococcus aureus to control translation of a human cytolytic peptide},
  author={Nour Sayed and Ambre Jousselin and Brice Felden},
  journal={Nature Structural \&Molecular Biology},
  year={2012},
  volume={19},
  pages={105-112}
}
Antisense RNAs (asRNAs) pair to RNAs expressed from the complementary strand, and their functions are thought to depend on nucleotide overlap with genes on the opposite strand. There is little information on the roles and mechanisms of asRNAs. We show that a cis asRNA acts in trans, using a domain outside its target complementary sequence. SprA1 small regulatory RNA (sRNA) and SprA1AS asRNA are concomitantly expressed in S. aureus. SprA1AS forms a complex with SprA1, preventing translation of… 

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References

SHOWING 1-10 OF 46 REFERENCES

cis-Antisense RNA, Another Level of Gene Regulation in Bacteria

TLDR
Emerging functional data suggest that asRNAs in bacteria mediate a plethora of effects and are involved in far more processes than were previously anticipated.

A search for small noncoding RNAs in Staphylococcus aureus reveals a conserved sequence motif for regulation

TLDR
This study unexpectedly shows that most of the novel ncRNAs carried in Staphylococcus aureus carry the conserved C−rich motif, suggesting that they are members of a class of nc RNAs that target mRNAs by a shared mechanism.

Experimental discovery of small RNAs in Staphylococcus aureus reveals a riboregulator of central metabolism

TLDR
A combination of biocomputational and transcriptional analyses revealed a remarkably coordinated RsaE-dependent downregulation of numerous metabolic enzymes involved in the citrate cycle and the folate-dependent one-carbon metabolism.

A Staphylococcus aureus Small RNA Is Required for Bacterial Virulence and Regulates the Expression of an Immune-Evasion Molecule

TLDR
It is demonstrated that SprD negatively regulates the expression of the Sbi immune-evasion molecule, impairing both the adaptive and innate host immune responses, and prevents translation initiation of sbi mRNA by an antisense mechanism.

A Small Bacterial RNA Regulates a Putative ABC Transporter*

TLDR
It is concluded that RydC regulates the yejABEF-encoded ABC permease at the mRNA level, and may contribute to optimal adaptation of some Enterobacteria to environmental conditions.

Small RNA genes expressed from Staphylococcus aureus genomic and pathogenicity islands with specific expression among pathogenic strains.

  • C. PichonB. Felden
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2005
TLDR
In vitro binding assays indicate that one sRNA encoded within a pathogenicity island is a trans-encoded antisense RNA regulating the expression of target genes at the posttranscriptional level, suggesting that they are involved in the regulation of staphylococcal virulence.

Bacterial antisense RNAs: how many are there, and what are they doing?

TLDR
What is known about the functions and mechanisms of action for some of these antisense RNAs and important considerations for future research as well as potential applications are discussed.

Regulatory RNAs in Bacteria

Identification of differentially expressed small non-protein-coding RNAs in Staphylococcus aureus displaying both the normal and the small-colony variant phenotype

TLDR
The presence of differentially expressed npcRNAs in S. aureus may help to understand the phenotypic variation and its associated pathogenicity.