A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence

  title={A chromosomally encoded type III secretion pathway in Yersinia enterocolitica is important in virulence},
  author={Jon C. Haller and Sharon J. Carlson and Kristin J. Pederson and Dorothy E. Pierson},
  journal={Molecular Microbiology},
Numerous Gram‐negative bacteria use a type III, or contact dependent, secretion system to deliver proteins into the cytosol of host cells. All of these systems identified to date have been shown to have a role in pathogenesis. We have identified 13 genes on the Yersinia enterocolitica chromosome that encode a type III secretion apparatus plus two associated putative regulatory genes. In order to determine the function of this chromosomally‐encoded secretion apparatus, we created an in frame… 
Transcriptional regulation of the Yts1 type II secretion system of Yersinia enterocolitica and identification of secretion substrates
Three proteins, ChiY, EngY and YE3650, that are secreted depending on a functional Yts1 T2SS in response to MgCl2 at low temperature are identified, demonstrating the need for additional Y. enterocolitica‐specific factors.
Bacterial secretion systems with an emphasis on the chlamydial Type III secretion system.
Current knowledge on the role of Type III secretion in the different stages of the chlamydial developmental cycle is summarized.
The fish pathogen Yersinia ruckeri possesses a TTS system.
A study was initiated to investigate the significance of TTSS to bacterial pathogens of fish and found no evidence of this plasmid in the fish pathogen Y. ruckeri.
Essential Role for Cyclic AMP and Its Receptor Protein in Yersinia enterocolitica Virulence
Results indicate that the cAMP-CRP regulatory system is required for Y. enterocolitica virulence and that cya and crp mutants to stimulate protective immunity against subsequent challenge with virulent bacteria in mice was promising.
A rationale for repression and/or loss of motility by pathogenic Yersinia in the mammalian host.
Artificial expression of flagellin in Y. enterocolitica completely attenuates virulence, supporting the hypothesis that motility is a liability in the mammalian host.
The Ysa type 3 secretion system of Yersinia enterocolitica biovar 1B.
  • G. Young
  • Biology, Medicine
    Advances in experimental medicine and biology
  • 2007
Investigation of the Ysa T3S system has revealed it is important for Y. enterocolitica survival during the gastrointestinal phase of infection, and targets the innate immune response to suppress the ability of the host to rapidly clear an infection.
Identification of in vivo-induced conserved sequences from Yersinia pestis during experimental plague infection in the rabbit.
The gathering of additional data and analysis of the intact IVI genes and the expressed IVICS products should provide insight into the unique biologic processes of Y. pestis during infection and reveal the genetic patterns of the pathogen's survival strategy in different hosts.
Environmental stimuli affecting expression of the Ysa type three secretion locus.
This study reports a new environmental stimulus for ysa gene expression; in the presence of high salt, growth on solid surface stimulates expression 7-fold compared to growth in high salt broth, indicating that, in the absence of salt, solid surface is an extremely robust signal for the Ysa system.
Lytic transglycosylases in macromolecular transport systems of Gram-negative bacteria
  • G. Koraimann
  • Biology
    Cellular and Molecular Life Sciences CMLS
  • 2003
Current knowledge on the role and distribution of these specialised murein-degrading enzymes in diverse macromolecular transport systems is summarised and discussed.


Identification of a virulence locus encoding a second type III secretion system in Salmonella typhimurium.
The newly identified SPI2 locus has a lower G+C content than that of the remainder of the Salmonella genome and is flanked by genes whose products share greater than 90% identity with those of the E. coli ydhE and pykF genes.
Type III secretion genes identify a putative virulence locus of Chlamydia
Four genes of Chlamydia psittaci strain guinea pig inclusion conjunctivitis, whose predicted products are highly homologous to structural and regulatory components of a contact‐dependent or type III secretion apparatus, were isolated and the possible role of this pathway in chlamydial pathogenesis is discussed.
Identification of two targets of the type III protein secretion system encoded by the inv and spa loci of Salmonella typhimurium that have homology to the Shigella IpaD and IpaA proteins
An important virulence factor of Salmonella spp. is their ability to gain access to host cells. A type III secretion system encoded in the inv and spa loci of these organisms is essential for this
Identification of a pathogenicity island required for Salmonella survival in host cells.
The "pathogenicity island" harboring the spi genes may encode the virulence determinants that set Salmonella apart from other enteric pathogens.
The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica
A gene expressed in the Bvg+ phase of Bordetella bronchiseptica that shows a high degree of sequence similarity to a locus involved in providing energy for type III secretion in pathogenic Gram‐negative bacteria (yscN in Yersinia spp.) is identified and regulated by bvg.
A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system.
The results suggest type III protein secretion by the flagellar system may be a general mechanism for the transport of proteins that influence bacterial-host interactions.
Type III Protein Secretion Systems in Bacterial Pathogens of Animals and Plants
  • C. Hueck
  • Biology
    Microbiology and Molecular Biology Reviews
  • 1998
A comparison of the structure, function, regulation, and impact on host cells of the type III secretion systems in the animal pathogens Yersinia spp.
Mutations affecting lipopolysaccharide enhance ail-mediated entry of Yersinia enterocolitica into mammalian cells
Two genes of Yersinia enterocolitica, inv and ail, have been identified as having a role in the bacterial adherence to and entry into mammalian cells in vitro and mutants that are increased in their ability to enter cells appear to be so as a result of a change in the LPS on the surface resulting in increased levels of Ail protein able to interact with the mammalian cell surface.
The hrp gene locus of Pseudomonas solanacearum, which controls the production of a type III secretion system, encodes eight proteins related to components of the bacterial flagellar biogenesis complex
The nucleotide sequence of the three other transcription units of the Pseudomonas solanacearum hrp gene cluster are presented, which are together predicted to code for 15 hrp genes, bringing the total number of Hrp proteins encoded by these five transcription units to 20, including HrPB, the positive regulatory protein, and HpaP, which is apparently not required for plant interactions.