A chromatographic method for the production of a human immunoglobulin G solution for intravenous use.

Abstract

Immunoglobulin G (IgG) of excellent quality for intravenous use was obtained from the cryosupernatant of human plasma by a chromatographic method based on a mixture of ion-exchange, DEAE-Sepharose FF and arginine Sepharose 4B affinity chromatography and a final purification step by Sephacryl S-300 HR gel filtration. The yield of 10 experimental batches produced was 3.5 g IgG per liter of plasma. A solvent/detergent combination of 1% Tri (n-butyl) phosphate and 1% Triton X-100 was used to inactivate lipid-coated viruses. Analysis of the final product (5% liquid IgG) based on the mean for 10 batches showed 94% monomers, 5.5% dimers and 0.5% polymers and aggregates. Anticomplementary activity was 0.3 CH50/mg IgG and prekallikrein activator levels were less than 5 IU/ml. Stability at 37 degrees C for 30 days in the liquid state was satisfactory. IgG was stored in flasks (2.5 g/flask) at 4 to 8 degrees C. All the characteristics of the product were consistent with the requirements of the 1997 Pharmacopée Européenne.

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Cite this paper

@article{Tanaka1998ACM, title={A chromatographic method for the production of a human immunoglobulin G solution for intravenous use.}, author={K Tanaka and E Sawatani and E M Shigueoka and T C Campos and H C Nakao and G A Dias and R K Fujita and F Arashiro}, journal={Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas}, year={1998}, volume={31 11}, pages={1375-81} }