A chromatin insulator protects retrovirus vectors from chromosomal position effects.

@article{Emery2000ACI,
  title={A chromatin insulator protects retrovirus vectors from chromosomal position effects.},
  author={David W. Emery and Evangelia Yannaki and Julie Tubb and G. Stamatoyannopoulos},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 16},
  pages={
          9150-5
        }
}
Recombinant murine retroviruses are widely used as delivery vectors for gene therapy. However, once integrated into a chromosome, these vectors often suffer from profound position effects, with vector silencing observed in vitro and in vivo. To overcome this problem, we investigated whether the HS4 chromatin insulator from the chicken beta-globin locus control region could protect a retrovirus vector from position effects. When used to flank a reporter vector, this element significantly… CONTINUE READING

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