A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.

@article{Weinmann1994ACT,
  title={A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.},
  author={P Weinmann and Martin Gossen and Wolfgang Hillen and Hermann Bujard and Christiane Gatz},
  journal={The Plant journal : for cell and molecular biology},
  year={1994},
  volume={5 4},
  pages={559-69}
}
The chimeric transcriptional activator tTA, a fusion between the Tn10 encoded Tet repressor and the activation domain of the Herpes simplex virion protein VP16, was stably expressed in transgenic tobacco plants. It stimulates transcription of the beta-glucuronidase (gus) gene from an artificial promoter consisting of 7 tet operators and a TATA-box. Tetracycline, which interferes with binding of tTA to operator DNA, reduces gus expression over several orders of magnitude. This stringency of… CONTINUE READING

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