A chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests

@article{Pernas1998ACS,
  title={A chestnut seed cystatin differentially effective against cysteine proteinases from closely related pests},
  author={M{\'o}nica Pernas and Rosa S{\'a}nchez-Monge and Lu{\'i}s G{\'o}mez and Gabriel Salcedo},
  journal={Plant Molecular Biology},
  year={1998},
  volume={38},
  pages={1235-1242}
}
Cystatin CsC, a cysteine proteinase inhibitor from chestnut (Castanea sativa) seeds, has been purified and characterized. Its full-length cDNA clone was isolated from an immature chestnut cotyledon library. The inhibitor was expressed in Escherichia coli and purified from bacterial extracts. Identity of both seed and recombinant cystatin was confirmed by matrix-assisted laser desorption/ionization mass spectrometry analysis, two-dimensional electrophoresis and N-terminal sequencing. CsC has a… CONTINUE READING

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