A chemically-defined medium for organotypic slice cultures.

  title={A chemically-defined medium for organotypic slice cultures.},
  author={Charles Annis and John Edmond and Richard T. Robertson},
  journal={Journal of neuroscience methods},
  volume={32 1},
Organotypic slice cultures provide an excellent system for the analysis of study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth. 

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