A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing.

@article{Liu2016ACC,
  title={A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing.},
  author={Kaiwen Ivy Liu and Muhammad Nadzim Bin Ramli and Cheok Wei Ariel Woo and Yuanming Wang and Tianyun Zhao and Xiujun Zhang and Guo Rong Daniel Yim and Bao Yi Chong and Ali Gowher and Mervyn Zi Hao Chua and Jonathan Jung and Jia Hui Jane Lee and Meng How Tan},
  journal={Nature chemical biology},
  year={2016},
  volume={12 11},
  pages={
          980-987
        }
}
CRISPR-Cas9 has emerged as a powerful technology that enables ready modification of the mammalian genome. The ability to modulate Cas9 activity can reduce off-target cleavage and facilitate precise genome engineering. Here we report the development of a Cas9 variant whose activity can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT) by fusing the Cas9 enzyme with the hormone-binding domain of the estrogen receptor (ERT2). The final optimized variant, termed iCas, showed low… CONTINUE READING

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