A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing.

@article{Liu2016ACC,
  title={A chemical-inducible CRISPR-Cas9 system for rapid control of genome editing.},
  author={Kaiwen Ivy Liu and Muhammad Nadzim Bin Ramli and Cheok Wei Ariel Woo and Yuanming Wang and Tian Yun Zhao and Xiujun Zhang and Guo Rong Daniel Yim and Bao Yi Chong and Ali Gowher and Mervyn Zi Hao Chua and Jonathan Jung and Jia Hui Jane Lee and Meng How Tan},
  journal={Nature chemical biology},
  year={2016},
  volume={12 11},
  pages={
          980-987
        }
}
CRISPR-Cas9 has emerged as a powerful technology that enables ready modification of the mammalian genome. The ability to modulate Cas9 activity can reduce off-target cleavage and facilitate precise genome engineering. Here we report the development of a Cas9 variant whose activity can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT) by fusing the Cas9 enzyme with the hormone-binding domain of the estrogen receptor (ERT2). The final optimized variant, termed iCas, showed low… 

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
The optimization of a drug-inducible CRISPR-Cas9 system that allows high-throughput gene interrogation with a temporal control and represents a significant upgrade on existing functional genomics toolbox is reported.

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TLDR
A perspective on advances in the precision control of Cas9 over aforementioned dimensions using external stimuli (e.g., small molecules or light) for controlled activation, inhibition, or degradation of Cas 9 is provided.

Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing

TLDR
HIT systems developed in this study can be applied for controlled modulation of potentially any genomic loci in multiple modes and exerted selective, titratable, rapid and reversible response to drug induction.
...

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