A centrifugation-free high-throughput protein purification system using in-line microfluidization.

Abstract

The majority of current high-throughput protein purification protocols include rate-limiting centrifugation steps. A column and nozzle assembly was developed that can be used in-line with microfluidization for the purification of bacterially-overexpressed, His-tagged proteins directly from bacterial cultures. Yields and purity are comparable with standard… (More)
DOI: 10.1016/j.pep.2011.05.006

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