A centrifugation-based method for extraction of norovirus from raspberries.

@article{Rzeutka2005ACM,
  title={A centrifugation-based method for extraction of norovirus from raspberries.},
  author={Artur Rzeżutka and Majed A. Alotaibi and Martin D'Agostino and Nigel T. Cook},
  journal={Journal of food protection},
  year={2005},
  volume={68 9},
  pages={1923-5}
}
A method was developed for extraction of noroviruses from raspberries. The method consists of removal of virus from fruit surfaces by alkaline extraction, then removal of residual food debris by centrifugation, followed by concentration of virus particles by ultracentrifugation. The efficiency of the extraction was estimated by comparison of reverse transcription PCR signals obtained from fruit extracts and the norovirus suspension that was used to contaminate the fruit. Noroviruses were… CONTINUE READING