A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.

@article{Tseng2017ACR,
  title={A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.},
  author={Chi-Kang Tseng and C S Chung and Hsin-Chou Chen and S Cheng},
  journal={RNA},
  year={2017},
  volume={23 4},
  pages={546-556}
}
Splicing of precursor mRNA occurs via two consecutive steps of transesterification reaction; both require ATP and several proteins. Despite the energy requirement in the catalytic phase, incubation of the purified spliceosome under proper ionic conditions can elicit competitive reversible transesterification, debranching, and spliced-exon-reopening… CONTINUE READING