A cell surface receptor complex for collagen type I recognizes the Arg- Gly-Asp sequence

@article{Dedhar1987ACS,
  title={A cell surface receptor complex for collagen type I recognizes the Arg- Gly-Asp sequence},
  author={Shoukat Dedhar and Erkki Ruoslahti and Michael D. Pierschbacher},
  journal={The Journal of Cell Biology},
  year={1987},
  volume={104},
  pages={585 - 593}
}
To isolate collagen-binding cell surface proteins, detergent extracts of surface-iodinated MG-63 human osteosarcoma cells were chromatographed on affinity matrices of either type I collagen-Sepharose or Sepharose carrying a collagen-like triple-helical peptide. The peptide was designed to be triple helical and to contain the sequence Arg-Gly-Asp, which has been implicated as the cell attachment site of fibronectin, vitronectin, fibrinogen, and von Willebrand factor, and is also present in type… CONTINUE READING