A cell-free culture system for development of large numbers of Brugia pahangi larvae.

@article{Hong1991ACC,
  title={A cell-free culture system for development of large numbers of Brugia pahangi larvae.},
  author={X Q Hong and Min Minnie Wong},
  journal={International journal for parasitology},
  year={1991},
  volume={21 8},
  pages={963-4}
}
Techniques used in the in vitro culture of massive numbers of Brugia pahangi third-stage larvae (L3) are described. Procedures for larval preparation and four culture conditions, with or without animal cell co-cultures, were studied, resulting in the adoption of a relatively simple cell-free culture system for routine harvesting of larval moulting excretory… CONTINUE READING