A cell-free assay using cytoplasmic cell extracts to study rejoining of radiation-induced DNA double-strand breaks in human cell nuclei.

Abstract

We describe a cell-free assay that can be employed to study rejoining of radiation-induced DNA double-strand breaks (dsbs) under in vitro conditions. The assay uses nuclei prepared from irradiated, agarose-embedded human A549 cells as substrate and cytoplasmic cell extracts prepared from exponentially growing HeLa cells as the source of enzymes. We… (More)

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