A cavity-containing mutant of T4 lysozyme is stabilized by buried benzene

@article{Eriksson1992ACM,
  title={A cavity-containing mutant of T4 lysozyme is stabilized by buried benzene},
  author={Annika Eriksson and W. A. Baase and Joan A. Wozniak and Brian W. Matthews},
  journal={Nature},
  year={1992},
  volume={355},
  pages={371-373}
}
THE hydrophobic cores of proteins are generally well packed, with few cavities1,2. Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein (our unpublished results). The sizes and shapes of such cavities can vary substantially depending on factors such as local geometry, whether or not a cavity already exists at the site of substitution, and the degree to which the protein structure… CONTINUE READING

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AlanineNo subtypeLeucine
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
We show by crystallographic and thermodynamic analysis that the cavity created by the replacement Leu 99 → Ala in T4 lysozyme is large enough to bind benzene and that ligand binding increases the melting temperature of the protein by 6.0 °C at pH 3.0 .
LeucineNo subtypeAlanine
We show by crystallographic and thermodynamic analysis that the cavity created by the replacement Leu 99 → Ala in T4 lysozyme is large enough to bind benzene and that ligand binding increases the melting temperature of the protein by 6.0 °C at pH 3.0 .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
Mutations in which a bulky buried residue such as leucine or phenylalanine is replaced with a small residue such as alanine can create cavities in the core of a protein ( our unpublished results ) .
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