A capture approach for supercoiled plasmid DNA using a triplex-forming oligonucleotide


Proteins that recognize and bind specific sites in DNA are essential for regulation of numerous biological functions. Such proteins often require a negative supercoiled DNA topology to function correctly. In current research, short linear DNA is often used to study DNA-protein interactions. Although linear DNA can easily be modified, for capture on a… (More)
DOI: 10.1093/nar/gkt239