A brief history of RNAi: the silence of the genes

@article{Sen2006ABH,
  title={A brief history of RNAi: the silence of the genes},
  author={George L. Sen and Helen M. Blau},
  journal={The FASEB Journal},
  year={2006},
  volume={20},
  pages={1293 - 1299}
}
  • G. Sen, H. Blau
  • Published 1 July 2006
  • Biology, Medicine
  • The FASEB Journal
The use of the RNA interference (RNAi) pathway to eliminate gene products has greatly facilitated the understanding of gene function. Behind this remarkable pathway is an intricate network of proteins that ensures the degradation of the target mRNA. In this review, we explore the history of RNAi as well as highlighting recent discoveries.—Sen, G. L., Blau, H. M. A brief history of RNAi: the silence of the genes. FASEB J. 20, 1293–1299 (2006) 

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References

SHOWING 1-10 OF 65 REFERENCES
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
TLDR
It is shown that rde-1 is a member of the piwi/sting/argonaute/zwille/eIF2C gene family conserved from plants to vertebrates and the possibility that one natural function of RNAi is transposon silencing is discussed. Expand
Assembly and function of RNA silencing complexes
  • E. Sontheimer
  • Biology, Medicine
  • Nature Reviews Molecular Cell Biology
  • 2005
TLDR
Advances have taken mechanistic understanding of RNA interference to a new level and promise to improve the ability to exploit this biological process for use in experimental biology and medicine. Expand
A large-scale RNAi screen in human cells identifies new components of the p53 pathway
TLDR
The construction of a set of retroviral vectors encoding 23,742 distinct shRNAs, which target 7,914 different human genes for suppression, is reported, which confers resistance to both p53-dependent and p19ARF-dependent proliferation arrest, and abolishes a DNA-damage-induced G1 cell-cycle arrest. Expand
A Link Between mRNA Turnover and RNA Interference in Arabidopsis
TLDR
It is proposed that mRNAs lacking a cap structure become exposed to RdRp to initiate or maintain RNAi, and it is shown that RdRP-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4. Expand
The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.
TLDR
Argonaute proteins comprise a highly conserved protein family that is involved in a variety of RNA silencing phenomena in a diverse set of organisms and may ultimately reveal the reach of these pathways into the basic biological mechanisms that underlie a range of human diseases. Expand
A species of small antisense RNA in posttranscriptional gene silencing in plants.
TLDR
The 25-nucleotide antisense RNA detected in transgene-induced PTGS is likely synthesized from an RNA template and may represent the specificity determinant of PTGS. Expand
Role for a bidentate ribonuclease in the initiation step of RNA interference
TLDR
Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs, and is evolutionarily conserved in worms, flies, plants, fungi and mammals, and has a distinctive structure, which includes a helicase domain and dualRNase III motifs. Expand
Argonaute2 Is the Catalytic Engine of Mammalian RNAi
TLDR
The evidence supports a model in which Argonaute contributes “Slicer” activity to RISC, providing the catalytic engine for RNAi. Expand
The enzymes and control of eukaryotic mRNA turnover
TLDR
It is highlighted that there are a variety of enzymes with different specificities, suggesting that individual nucleases act on distinct subpopulations of transcripts within the cell and multiple mechanisms by which mRNA degradation could be regulated. Expand
Genetic requirements for inheritance of RNAi in C. elegans.
TLDR
Findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and r de-4 in the formation of the inherited agent. Expand
...
1
2
3
4
5
...