A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml.

@article{Collins1997ABD,
  title={A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml.},
  author={M. L. Collins and Bruce Irvine and D Tyner and Erika Fine and C L Zayati and Chih-Pei Chang and Thomas Horn and D Ahle and J. Detmer and Luping Shen and Janice A. Kolberg and Steven Bushnell and M. S. Urdea and David D. Ho},
  journal={Nucleic acids research},
  year={1997},
  volume={25 15},
  pages={2979-84}
}
The branched DNA hybridization assay has been improved by the inclusion of the novel nucleotides, isoC and isoG, in the amplification sequences to prevent non-specific hybridization. The novel isoC, isoG-containing amplification sequences have no detectable interaction with any natural DNA sequence. The control of non-specific hybridization in turn permits increased signal amplification. Addition of a 14 site preamplifier was found to increase the signal/noise ratio 8-fold. A set of 74… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 87 extracted citations

Advanced Techniques in Diagnostic Microbiology

Springer US • 2013
View 10 Excerpts
Highly Influenced

References

Publications referenced by this paper.

Nucleosides Nucleotides 10, 389–392

Urdea, B.D.M.S. Warner, +3 authors T. Horn
Nucleic Acids Res • 1995

Similar Papers

Loading similar papers…