A boronate affinity sandwich assay: an appealing alternative to immunoassays for the determination of glycoproteins.

@article{Ye2014ABA,
  title={A boronate affinity sandwich assay: an appealing alternative to immunoassays for the determination of glycoproteins.},
  author={Jin Ye and Yang Chen and Zhen Liu},
  journal={Angewandte Chemie},
  year={2014},
  volume={53 39},
  pages={10386-9}
}
Immunoassay has been an essential tool in many areas, including clinical diagnostics. However, it suffers from drawbacks, such as poor availability of high specificity antibodies, limited stability of biological reagents, as well as damage to health and susceptibility of chemical labels to the sample environment. Here we present a new approach, a boronate-affinity sandwich assay (BASA), for the specific and sensitive determination of trace glycoproteins in complex samples. BASA relies on the… CONTINUE READING
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