A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein.

@article{Okoh2006ABF,
  title={A biosensor for inorganic phosphate using a rhodamine-labeled phosphate binding protein.},
  author={Michael P. Okoh and Jackie L. Hunter and John E. T. Corrie and Martin R Webb},
  journal={Biochemistry},
  year={2006},
  volume={45 49},
  pages={14764-71}
}
A novel biosensor for inorganic phosphate (Pi) has been developed based on the phosphate binding protein of Escherichia coli. Two cysteine mutations were introduced and labeled with 6-iodoacetamidotetramethylrhodamine. When physically close to each other and correctly oriented, two rhodamine dyes interact to form a noncovalent dimer. In this state, they have little or no fluorescence, unlike the high fluorescence intensity of monomeric rhodamine. The labeling sites were so placed that the… CONTINUE READING

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