A bioluminescence assay to detect nitrification inhibitors released from plant roots: a case study with Brachiaria humidicola

@article{Subbarao2006ABA,
  title={A bioluminescence assay to detect nitrification inhibitors released from plant roots: a case study with Brachiaria humidicola},
  author={G. Subbarao and T. Ishikawa and O. Ito and K. Nakahara and H. Wang and W. Berry},
  journal={Plant and Soil},
  year={2006},
  volume={288},
  pages={101-112}
}
A bioluminescence assay using recombinant Nitrosomonas europaea was adopted to detect and quantify natural nitrification inhibitors in plant–soil systems. The recombinant strain of N. europaea produces a distinct two-peak luminescence due to the expression of luxAB genes, introduced from Vibrio harveyi, during nitrification. The bioluminescence produced in this assay is highly correlated with NO2− production (r2 = 0.94). Using the assay, we were able to detect significant amounts of a… Expand

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References

SHOWING 1-10 OF 32 REFERENCES
A Bioluminescence Assay Using Nitrosomonas europaeafor Rapid and Sensitive Detection of Nitrification Inhibitors
TLDR
It is concluded that the assay system using luminous Nitrosomonas can be applied as a rapid and sensitive detection test for nitrification inhibitors, and it will be used to monitor the nitrification process in wastewater treatment plants. Expand
Suppression of nitrification and nitrous oxide emission by the tropical grass Brachiaria humidicola
Nitrification by soil nitrifiers may result in substantial losses of applied nitrogen through NO3 − leaching and N2O emission. The biological inhibition of nitrification by crop plants or pastureExpand
Inhibition of Nitrosomonas europaea by Monoterpenes from Coastal Redwood (Sequoia sempervirens) in Whole-Cell Studies
Inhibition by allelochemicals, including monoterpenes, has been suggested as a factor in the extremely low nitrification rates observed in coastal redwood forests. Similarities between the molecularExpand
Mineral nitrogen in an oxisol from the Brazilian cerrados in the presence of Brachiaria spp.
TLDR
It is concluded that the species studied had different rates of N uptake and assimilation and stimulated microorganisms differently in their rhizosphere, which lead to contrasting transformations on the soil mineral N pool. Expand
Repression of nitrification in soils under a climax grassland vegetation
Two hypotheses on repression of nitrification in climax vegetations (i.e. nitrogen immobilization and allelopathy) were investigated. In this study the potential nitrification activities and numbersExpand
Inhibition of nitrification in forest soil by monoterpenes
Nitrate production was detected in untreated soil of a Norway spruce (Picea abies L.) stand only after clear-cutting the stand. The aim of this study was to determine whether allelochemicalExpand
The role of monoterpenes in soil nitrogen cycling processes in ponderosa pine
The effects of select monoterpenes on nitrogen (N) mineralization and nitrification potentials were determined in four separate laboratory bioassays. The effect of increasing monoterpene addition wasExpand
Stable coexistence of contrasted nitrification statuses in a wet tropical savanna ecosystem
TLDR
By comparing two sites in the Lamto area (Cote d’Ivoire, West Africa) with similar soil physico-chemical characteristics and equally dominated by H. diplandra, it was demonstrated that nitrification is highly heterogeneous, with a 240-fold variation in potential nitrification within a specific site. Expand
Grass populations control nitrification in savanna soils
TLDR
Results suggest that plant species can have important consequences for N cycling at the population level, as the denitrifying enzyme activity (DEA) is much higher in the high-nitrification site than in the low-nitrifying site. Expand
NATURALLY OCCURRING IRON-CHELATING COMPOUNDS IN OAT-AND RICE-ROOT WASHINGS : I. Activity Measurement and Preliminary Characterization
Abstract The root washings of water-cultured oat and rice (non-sterile) contained some sort of amphoteric, iron-solubilizing chelating (or complexing) compound(s), which could be separated into aExpand
...
1
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3
4
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