• Corpus ID: 34986464

A biochemical investigation into the inhibitory effects of glucosamine and N-acetylclucosamine on the aggregation in vitro of embryonic chick muscle cells.

@article{Lloyd1971ABI,
  title={A biochemical investigation into the inhibitory effects of glucosamine and N-acetylclucosamine on the aggregation in vitro of embryonic chick muscle cells.},
  author={Clive Lloyd and Richard B. Kemp},
  journal={Journal of cell science},
  year={1971},
  volume={9 1},
  pages={
          85-101
        }
}
  • C. LloydR. Kemp
  • Published 1 July 1971
  • Biology, Chemistry
  • Journal of cell science
The effect of glucosamine and N -acetylglucosamine on aggregation and energy metabolism was investigated over an 8-h period in cells dissociated by 0.25% (w/v) trypsin from the skeletal muscle of 9-day-old chick embryos. At 8 h, 0.023 M glucosamine and N -acetylglucosamine inhibited the aggregation of cells suspended in Eagle9s minimal essential medium by 18.9% and 16.4% respectively, as judged on a basis of aggregate size. Glucosamine and N -acetylglucosamine reduced the cellular ATP level by… 

Effects of cytochalasins on the initial aggregation in vitro of embryonic chick cells.

The effect of the cytochalasins on aggregate structure was interpreted on the basis of arrested cellular motility, but the singular inhibition by cy tochalasin A of the rate of aggregation must await final confirmation of its site of action.

The role of extracellular materials in cell movement. I. Inhibition of mucopolysaccharide synthesis does not stop ruffling membrane activity or cell movement

The results suggest that if mucopolysaccharides are required for cell movement, they must have long half-lives or represent only a minute fraction of the normal synthetic load.

Microcalorimetric studies of tissue cells and bacteria

The present results underline the value of microcalorimetry in giving analytical data on growth and respiration in cells and organisms and illustrates the potential of the technique in studies of surface-localised reactions.

Intercellular adhesive specificity.

References

SHOWING 1-10 OF 33 REFERENCES

Inhibitory effect of D-glucosamine and other sugars on the biosynthesis of protein, ribonucleic acid, and deoxyribonucleic acid in normal and neoplastic tissues.

The effect of exogenous glucosamine in vitro on the incorporation of the labeled precursors into normal tissues was much less than that into neoplastic tissues.

The biosynthesis of glycoproteins. V. Incorporation of glucosamine-1-14 C into macromolecules by Ehrlich ascites carcinoma cells.

Glucosamine-14C was rapidly incorporated by Ehrlich ascites carcinoma cells into trichloroacetic acid-insoluble molecules both in vivo and in vitro, and among the subcellular fractions, the microsomes were the most highly labeled.

Metabolism of D-glucosamine and N-acetyl-D-glucosamine in the intact rat.

Studies on the mechanism underlying the inhibition by puromycin of cell aggregation in vitro.

The effects of puromycin on the aggregation, protein synthesis and cellular metabolism of trypsin-dissociated cells are discussed in relation to cellular adhesive mechanisms.

The effect of neuraminidase (3:2:1:18) on the aggregation of cells dissociated from embryonic chick muscle tissue.

  • R. Kemp
  • Biology
    Journal of cell science
  • 1970
Embryonic chick muscle cells were used to investigate the effect of removing cell-surface sialic acids on cell aggregation in vitro and Interpretation of the results in the light of current theories of cell adhesion failed to give support to the concept of adhesion by physical forces.

Inhibitory effect of d-glucosamine and other sugar analogs on the viability and transplantability of ascites tumor cells.

d-Glucosamine has been shown to have a powerful cytotoxic effect on various ascites tumor lines, resulting in a decrease in viability and transplantability of the neoplastic cells, and d-Mannose was the only neutral sugar showing a cytot toxic effect on ascite tumor cells.