A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production

  title={A bioassay for brassinosteroid activity based on the in vitro fluorimetric detection of nitric oxide production},
  author={Vanesa E. Tossi and Sof{\'i}a L. Acebedo and Ra{\'u}l Oscar Cassia and Lorenzo Lamattina and Lydia R. Galagovsky and Javier Alberto Ramirez},
Brassinosteroids Induce Strong, Dose-Dependent Inhibition of Etiolated Pea Seedling Growth Correlated with Ethylene Production
It is demonstrated here that the growth inhibition of etiolated pea shoots strongly correlates with increases in ethylene production, which also responds dose-dependently to applied BRs.
Nitric oxide is involved in the brassinolide-induced adventitious root development in cucumber (Cucumis sativus L.) explants
Background: Brassinolide (BR), as a new type of plant hormones, is involved in the process of plant growth and stress response. Previous studies have reported the roles of BR in regulating plant


Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen
The isolation, structure determination and biological activity of this new plant growth promoter, named (22R, 23R, 24S)-2α-3α,22,23-tetrahydroxy-24-methyl-6,7-s-5α-cholestano-6-7-lactone or brassinolide is reported.
Brassinosteroid phytohormones: structure, bioactivity and applications
Brassinosteroids are a new class of plant hormones with a polyoxygenated steroid structure showing pronounced plant growth regulatory activity and prospective agricultural uses.
Pharmacological and genetical evidence supporting nitric oxide requirement for 2,4-epibrassinolide regulation of root architecture in Arabidopsis thaliana
It is demonstrated that BR increases the NO concentration in root cells, which is required for BR-induced changes in root architecture, in Arabidopsis seedlings treated with BR 24-epibrassinolide.
Metabolism of brassinosteroids in plants.
  • A. Bajguz
  • Chemistry, Biology
    Plant physiology and biochemistry : PPB
  • 2007
Structure–Activity Studies of Brassinosteroids and the Search for Novel Analogues and Mimetics with Improved Bioactivity
A number of novel brassinosteroid analogues were synthesized and subjected to the rice leaf lamina inclination bioassay, finding that the compound where three of the four hydroxyl groups had been converted to methyl ethers retained substantial bioactivity.
Brassinosteroid action in flowering plants: a Darwinian perspective.
'Nothing in brassinosteroid research makes sense except in the light of Darwinian evolution' is concluded and the value of basic science is highlighted, with reference to the genetic engineering of better food crops that may become resistant to a variety of plant diseases.
Brassinosteroids: A New Class of Plant Hormones
This chapter discusses Brassionsteroids in Nature, basic Synthetic Methods and Formal Synthesis, Practical Applications and Toxicology, and Bioassays and Structure Activity Relationship of BS.