A binding site for Gli proteins is essential for HNF - 3 β floor plate enhancer activity in transgenics and can respond to Shh in vitro

Abstract

The floor plate is a group of specialized cells located in the ventral midline of the vertebrate neural tube. It plays important roles in neural tube development as a source of signalling molecules for dorsoventral patterning and axonal guidance (for reviews, see Jessell and Dodd, 1992; Dodd and Jessell, 1993). A floor plate-derived signalling molecule, Sonic hedgehog (Shh), which is a vertebrate homologue of Drosophila Hedgehog (Hh), induces differentiation of ventral type neurons (Ericson et al., 1995; Marti et al., 1995; Roelink et al., 1995). Another floor plate-derived signalling molecule, netrin-1, works as both a chemoattractant and a chemorepellent of axons, depending on neuronal cell types (Kennedy et al., 1994; Colamarino and Tessier-Lavigne, 1995). Differentiation of the floor plate is induced by a signal derived from the notochord or by Shh. High concentrations of Shh-N peptide or contact with the notochord are required for floor plate induction in neural explants (Marti et al., 1995; Roelink et al., 1995). A critical event of floor plate development is the expression of a winged-helix transcription factor, HNF-3β (Ang et al., 1993; Ruiz i Altaba et al., 1993; Sasaki and Hogan, 1993, 1994). Ectopic expression of HNF-3β in the neural tube induces ectopic expression of series of floor plate marker genes and prevents dorsal/lateral maker expression, indicating that HNF-3β acts as a regulator of floor plate development (Sasaki and Hogan, 1994; Ruiz i Altaba et al., 1995a). The HNF-3β expression in the floor plate is induced by a signal derived from the notochord or by Shh, and its induction does not require de novo protein synthesis (Roelink et al., 1995; Ruiz i Altaba et al., 1995b; Chiang et al., 1996). This makes HNF-3β a candidate for a direct target of Shh signalling. Previously, we have identified cis-regulatory regions of the mouse HNF-3β gene in transgenic mouse embryos. Enhancers for floor plate expression and node/notochord expression were 1313 Development 124, 1313-1322 (1997) Printed in Great Britain © The Company of Biologists Limited 1997 DEV4800

6 Figures and Tables

050100'98'00'02'04'06'08'10'12'14'16
Citations per Year

446 Citations

Semantic Scholar estimates that this publication has 446 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@inproceedings{Sasaki1997ABS, title={A binding site for Gli proteins is essential for HNF - 3 β floor plate enhancer activity in transgenics and can respond to Shh in vitro}, author={Hiroshi Sasaki and Chi - chung Hui and Masato Nakafuku and Hisato Kondoh}, year={1997} }