A binding-site-deficient, catalytically active, core protein of endoglucanase III from the culture filtrate of Trichoderma reesei.

@article{Sthlberg1988ABC,
  title={A binding-site-deficient, catalytically active, core protein of endoglucanase III from the culture filtrate of Trichoderma reesei.},
  author={Jerry St{\aa}hlberg and Gunnar Johansson and Gunilla Pettersson},
  journal={European journal of biochemistry},
  year={1988},
  volume={173 1},
  pages={179-83}
}
From the culture filtrate of Trichoderma reesei we have isolated a novel endoglucanase (38 kDa) which was shown to be identical to endoglucanase III (E III, 50 kDa), but lacking the first 61 N-terminal amino acids. This core protein, designated E III core, is fully active against soluble substrates, such as carboxymethylcellulose, whereas both activity against and adsorption to microcrystalline cellulose (Avicel) is markedly decreased. Sedimentation velocity experiments revealed that the intact… CONTINUE READING