Molecular and enzymatic studies of cyclodextrin glucanotransferase gene from Bacillus sp. TS1-1
Sago starch was found to be the best substrate for cyclodextrin (CD) production among other starch sources (corn, rice, soluble and tapioca starch) and only I²- and I³-CD were detected during the production of CDs.
Engineering reaction and product specificity of cyclodextrin glycosyltransferase from Bacillus circulans strain 251
Engineering of cyclodextrin glycosyltransferase reaction and product specificity.
- BiologyBiochimica et biophysica acta
What makes cyclodextrin glycosyltransferase a transglycosylase
All living organisms need energy from their environment for maintenance and growth, therefore, these organisms secrete several starch degrading enzymes of which cyclodextrin glycosyltransferase (CGTase) is an example.
Properties and applications of starch-converting enzymes of the α-amylase family
Rational design of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 to increase alpha-cyclodextrin production.
- Biology, ChemistryJournal of molecular biology
Hydrogen bonds (S146) and hydrophobic contacts (Y89) appear to contribute strongly to the size of cyclodextrin products formed and thus to CGTase product specificity, and changes in sugar binding subsites -3 and -7 thus result in mutant proteins with changed cyclodesxtrin production specificity.
Starch Modification and Application
Starch occurs widely in nature and is the second largest biomass on earth after cellulose and one of the most abundant bio-renewable materials. The properties of native starch do not always meet the…
Starch Structure, Functionality and Application in Foods
This chapter presents a brief overview of key discoveries that led to our current knowledge of starch. Due to the variability of natural starch, and the significance of this biopolymer to humans in…
Production and application of α-amylase from indigenous fungal strain Aspergillus luchuensis bs 1
A novel indigenous fungal strain having ability to produce alpha amylase and an enzyme preparation for desizing sized cotton fabric in minimal concentration is proposed.
Screening and Selection of Medium Components for Cyclodextrin Glucanotransferase Production by New Alkaliphile Microbacterium terrae KNR 9 Using Plackett-Burman Design
- Biology, MedicineBiotechnology research international
Cyclodextrin glucanotransferase (CGTase, EC 184.108.40.206) production using new alkaliphile Microbacterium terrae KNR 9 was investigated by submerged fermentation. Statistical screening for components…