A benchmarking of workflows for detecting differential splicing and differential expression at isoform level in human RNA-seq studies

@article{Merino2017ABO,
  title={A benchmarking of workflows for detecting differential splicing and differential expression at isoform level in human RNA-seq studies},
  author={Gabriela Alejandra Merino and Ana Conesa and Elmer Andr{\'e}s Fern{\'a}ndez},
  journal={bioRxiv},
  year={2017}
}
Over the last few years, RNA-seq has been used to study alterations in alternative splicing related to several diseases. [] Key Method We evaluated the workflows performance over different experimental scenarios where changes in absolute and relative isoform expression occurred simultaneously. In addition, the effect of the number of isoforms per gene, and the magnitude of the expression change over pipeline performances were also evaluated. Our results suggest that workflow performance is influenced by the…

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