A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry.

@article{Spiro2000ABM,
  title={A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry.},
  author={Alexander Spiro and Mary Lowe and Db Brown},
  journal={Applied and environmental microbiology},
  year={2000},
  volume={66 10},
  pages={4258-65}
}
A new multiplexed, bead-based method which utilizes nucleic acid hybridizations on the surface of microscopic polystyrene spheres to identify specific sequences in heterogeneous mixtures of DNA sequences is described. The method consists of three elements: beads (5.6-microm diameter) with oligomer capture probes attached to the surface, three fluorophores for multiplexed detection, and flow cytometry instrumentation. Two fluorophores are impregnated within each bead in varying amounts to create… CONTINUE READING

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