A bead-based approach for large-scale identification of in vitro kinase substrates.

@article{Zhang2011ABA,
  title={A bead-based approach for large-scale identification of in vitro kinase substrates.},
  author={Manyu Zhang and Guanghui Han and Chunli Wang and Kai Cheng and Ruibin Li and Hongwei Liu and Xiaoluan Wei and Mingliang Ye and Hanfa Zou},
  journal={Proteomics},
  year={2011},
  volume={11 24},
  pages={4632-7}
}
Deciphering the kinase-substrate relationship is vital for the study of phosphorylation network. The use of immobilized proteins on protein chip as the library for screening of potential kinase substrates is a tried-and-tested method. However, information on phosphorylation sites is lacking and the creation of the library with proteins of whole proteome by recombinant expression is costly and difficult. In this study, a new solid-phase approach by immobilization of proteins from cell lysate… CONTINUE READING
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