A bacterial artificial chromosome (BAC) library for sunflower, and identification of clones containing genes for putative transmembrane receptors

@article{Gentzbittel2001ABA,
  title={A bacterial artificial chromosome (BAC) library for sunflower, and identification of clones containing genes for putative transmembrane receptors},
  author={Laurent Gentzbittel and Albert E. Abbott and J. P. Galaud and Laura Georgi and Francis Fabre and Thierry Liboz and Gilbert Alibert},
  journal={Molecular Genetics and Genomics},
  year={2001},
  volume={266},
  pages={979-987}
}
Sunflower (Helianthus annuus L.) is an economically important oil seed crop with an estimated genome size of 3000 Mb. We have constructed a bacterial artificial chromosome (BAC) library for sunflower, which represents an estimated 4- to 5-fold coverage of the genome. Nuclei isolated from young leaves were used as a source of high-molecular-weight DNA and a partial restriction endonuclease digestion protocol was used to cleave the DNA. A random sample of 60 clones indicated an average insert… CONTINUE READING
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