A Yeast Metabolite Extraction Protocol Optimised for Time-Series Analyses

  title={A Yeast Metabolite Extraction Protocol Optimised for Time-Series Analyses},
  author={Kalesh Sasidharan and Tomoyoshi Soga and Masaru Tomita and Douglas B. Murray},
  booktitle={PloS one},
There is an increasing call for the absolute quantification of time-resolved metabolite data. However, a number of technical issues exist, such as metabolites being modified/degraded either chemically or enzymatically during the extraction process. Additionally, capillary electrophoresis mass spectrometry (CE-MS) is incompatible with high salt concentrations often used in extraction protocols. In microbial systems, metabolite yield is influenced by the extraction protocol used and the cell… CONTINUE READING
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