A Yeast Genetic Screen Reveals a Critical Role for the Pore Helix Domain in TRP Channel Gating

@article{Myers2008AYG,
  title={A Yeast Genetic Screen Reveals a Critical Role for the Pore Helix Domain in TRP Channel Gating},
  author={B. R. Myers and Christopher J. Bohlen and David Julius},
  journal={Neuron},
  year={2008},
  volume={58},
  pages={362-373}
}
TRP cation channels function as cellular sensors in uni- and multicellular eukaryotes. Despite intensive study, the mechanisms of TRP channel activation by chemical or physical stimuli remain poorly understood. To identify amino acid residues crucial for TRP channel gating, we developed an unbiased, high-throughput genetic screen in yeast that uncovered rare, constitutively active mutants of the capsaicin receptor, TRPV1. We show that mutations within the pore helix domain dramatically increase… CONTINUE READING
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The use of yeast to understand TRP-channel mechanosensitivity

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