A Versatile Medium for Cultivating Methanogenic Archaea

  title={A Versatile Medium for Cultivating Methanogenic Archaea},
  author={Saber Khelaifia and Didier Raoult and Michel Drancourt},
  booktitle={PloS one},
BACKGROUND Methanobrevibacter smithii, Methanobrevibacter oralis, Methanosphaera stadtmanae, Methanomassilicoccus luminyensis and Methanobrevibacter arboriphilicus have been cultured from human digestive microbiota. Each one of these fastidious methanogenic archaea requires a specific medium for its growth, hampering their routine isolation and the culture. METHODOLOGY/PRINCIPAL FINDINGS A new culture medium here referred as SAB medium was optimized and tested to cultivate methanogens… CONTINUE READING
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