A Validated HPTLC Densitometric Method for Simultaneous Determination of Evernic and Usnic Acids in Four Usnea Species and Comparison of Their Antioxidant Potential.

@article{Singh2016AVH,
  title={A Validated HPTLC Densitometric Method for Simultaneous Determination of Evernic and Usnic Acids in Four Usnea Species and Comparison of Their Antioxidant Potential.},
  author={S. Singh and S. Khatoon and Y. Joshi and Siddhartha Prgyadeep and D. Upreti and A. Rawat},
  journal={Journal of chromatographic science},
  year={2016}
}
A simple, sensitive and precise high-performance thin-layer chromatography method with densitometric detection was used for simultaneous determination of evernic (EV) and (+)-usnic acids (USN) in Usnea aciculifera (UA), U. ghattensis (UG), U. longissima (UL) and U. stigmatoides (US). This method was also validated according to the ICH guidelines. Separation and quantification was performed with the mobile phase toluene-1, 4-dioxane-formic acid (18:4.5:0.2, v/v/v) on silica gel 60F254 plates… Expand
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