Corpus ID: 27914735

A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF BETULIN, LUPEOL AND STIGMASTEROL IN ASTERACANTHA LONGIFOLIA NEES

@inproceedings{Maji2014AVR,
  title={A VALIDATED RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF BETULIN, LUPEOL AND STIGMASTEROL IN ASTERACANTHA LONGIFOLIA NEES},
  author={Amal K. Maji and Subrata Pandit and Pratim Banerji and Debdulal Banerjee},
  year={2014}
}
Objective: The aim of the present study was to develop a reversed phase high-performance liquid chromatography (RP-HPLC) method for simultaneous estimation of betulin, lupeol and stigmasterol in Asteracantha longifolia extract. Methods: The chromatographic separation was achieved using Luna C18 column (5 µm, 250 x 4.6 mm) under isocratic elution of acetonitrile and 0.1% acetic acid in water (94: 6, v/v) with a flow rate of 1.0 mL min -1 and the detection wavelength was set at 215 nm. The column… Expand

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References

SHOWING 1-10 OF 14 REFERENCES
Simultaneous quantitation of lupeol and ï¢-sitosterol from the whole plant powder of Asteracantha longifolia Nees
A simple, sensitive and accurate high performance thin layer chromatographic method has been established for simultaneous quantitation of Lupeol and i¢-sitosterol in the whole plant powder ofExpand
RP-HPLC-DAD for simultaneous estimation of mahanine and mahanimbine in Murraya koenigii.
TLDR
A rapid reverse-phase high-performance liquid chromatography (RP-HPLC) method has been developed for quantitative estimation of mahanine and mahanimbine, two major bioactive alkaloids in this plant. Expand
Simultaneous Determination of Biological Marker Compounds in Ostericum koreanum by HPLC Method and Discrimination by Principal Component Analysis
An analytical method for the simultaneous determination of four biological marker compounds including bisabolangelone, oxypeucedanin, imperatorin, and isoimperatorin extracted from Ostericum koreanumExpand
Simultaneous estimation of three triterpenoids from Carissa carandas using validated high performance liquid chromatography
TLDR
The HPLC method could be an effective technique for routine analysis of lupeol, stigmasterol, and b-sitosterol simultaneously and monitoring the quality of fruit and its available products in cosmetic and pharmaceutical companies. Expand
Simultaneous determination of betulin and betulinic acid in white birch bark using RP-HPLC.
TLDR
It was found 95% ethanol was a good extraction solvent that allowed extraction of triterpenoid with a highest content and significant variations in the content of betulin and betulinic acid in white birch bark growing in different locations of China. Expand
The role of matrix effects on the quantification of abscisic acid and its metabolites in the leaves of Bauhinia variegata L. using liquid chromatography combined with tandem mass spectrometry
Phytohormone analysis using liquid chromatography combined with tandem mass spectrometry is a very important tool for studies involving plant metabolism; however, this analysis can be affected byExpand
Separation and identification of some common isomeric plant triterpenoids by thin-layer chromatography and high-performance liquid chromatography.
TLDR
The combination of 3 TLC methods and 2 HPLC methods enables qualitative determination of all 12 compounds and proves to be useful for the analysis of plant extracts. Expand
Supercritical fluid extraction of Alnus glutinosa (L.) Gaertn.
Abstract Supercritical carbon dioxide extraction of Alnus glutinosa (L.) Gaertn. was performed ( P  = 300/450 bar, T  = 40/60 °C, EtOH addition = 0/5/10%) in order to determine preferable processExpand
Systematic and comprehensive strategy for reducing matrix effects in LC/MS/MS analyses.
TLDR
The combination of polymeric mixed-mode SPE, the appropriate mobile phase pH and UPLC technology provides significant advantages for reducing matrix effects resulting from plasma matrix components and in improving the ruggedness and sensitivity of bioanalytical methods. Expand
Pharmacological properties of the ubiquitous natural product betulin.
TLDR
A new mechanism of action has been confirmed for some of the most promising anti-HIV derivatives, which makes them potentially useful additives to the current anti- HIV therapy. Expand
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